16 RT-PCR

I-Loop-mediated isothermal amplification (LAMP) ubuchwepheshe obusha bokukhulisa izakhi zofuzo, nohlobo olusha lwendlela yokukhulisa i-nucleic acid, isici sayo ukuthi izinhlobo ezi-4 zezinhlobo ezithile zenzelwe izifunda ezi-6 zesakhi sofuzo, ngaphansi kwesenzo se-Bst DNA i-polymerase, i-60 ~ 65 ampl isothermal amplification, ingafinyelela ku-109 ~ 1010 izikhathi zokukhulisa i-nucleic acid cishe imizuzu eyi-15 kuye kwengu-60, inezici zokusebenza okulula, ukucaciswa okuqinile nokutholwa komkhiqizo okulula.

I-MA-1610 uhlelo oluvulekile olwenzelwe i-isothermal fluorescence PCR olunemigqa emibili yokuthola ukutholakala kwe-8 × 0.2ml ne-3-color fluorescence ukuthola isiteshi, esuselwa ekusetshenzisweni kweLAMP futhi kuhlanganiswe nobuchwepheshe bokubona i-fluorescence. ukucaciswa, ukuzwela okuphezulu, ubulula, ukusebenziseka kalula kanye nezindleko eziphansi, kusetshenziswe kabanzi ekuhlolweni kwezifo zemitholampilo, ukutholwa kwekhwalithi / okulinganayo kwamabhaktheriya wesifo esiwumshayabhuqe / amagciwane, ukukhonjwa kobulili kwemibungu yezilwane nokwakhiwa kwama-chip chips.